Nasr El-Din, W., Hassan, G., Abdel-Hamed, A., Kamel, A. (2013). Effect of Exogenous Interleukin-10 on Carbon Tetrachloride CCL4-Induced Hepatic Fibrosis in Rats. Suez Canal University Medical Journal, 16(1), 29-36. doi: 10.21608/scumj.2013.45657
Wael Nasr El-Din; Gamal M Hassan; Alaa El-Din S Abdel-Hamed; Amr A Kamel. "Effect of Exogenous Interleukin-10 on Carbon Tetrachloride CCL4-Induced Hepatic Fibrosis in Rats". Suez Canal University Medical Journal, 16, 1, 2013, 29-36. doi: 10.21608/scumj.2013.45657
Nasr El-Din, W., Hassan, G., Abdel-Hamed, A., Kamel, A. (2013). 'Effect of Exogenous Interleukin-10 on Carbon Tetrachloride CCL4-Induced Hepatic Fibrosis in Rats', Suez Canal University Medical Journal, 16(1), pp. 29-36. doi: 10.21608/scumj.2013.45657
Nasr El-Din, W., Hassan, G., Abdel-Hamed, A., Kamel, A. Effect of Exogenous Interleukin-10 on Carbon Tetrachloride CCL4-Induced Hepatic Fibrosis in Rats. Suez Canal University Medical Journal, 2013; 16(1): 29-36. doi: 10.21608/scumj.2013.45657
Effect of Exogenous Interleukin-10 on Carbon Tetrachloride CCL4-Induced Hepatic Fibrosis in Rats
1Department of Anatomy, Faculty of Medicine, Suez Canal University, Egypt.
2Department of Clinical Pathology, Faculty of Medicine, Suez Canal University, Egypt.
3Departments of Pathology, Faculty of Medicine, Suez Canal University, Egypt.
Abstract
Background: Transforming growth factor β1 (TGF-β1) is one of the strongest profibrotic cytokines and TGF-β1/Smad signaling is the cardinal signal transduction pathway involved in fibrosis. Previous studies indicated that exogenous IL-10 down regulates collagen type I. It also exerts antifibrogenic effect by down regulating profibrogenic cytokines such as (TGF- β1). All these studies indicate that IL-10 might become a new therapeutic target. Aim: To investigate the potential therapeutic effect of exogenous interleukin-10 on reversing the well-established hepatic fibrosis of CCl4 administration in experimental rats.Materials and Methods: Albino rats were divided into four groups (27 rats each): group A (control), group B1 (CCl4-treated), group B2 (spontaneous recovery, SR) and group B3 (IL-10-treated). Rats' liver tissue was stained with i) hematoxylin and eosin (H & E) and, ii) Masson’s trichrome stains for evaluation of the histological activity index (HAI). Serum TGF-β1 was determined by ELISA. Results:No inflammation was found in the control group, however, marked inflammation (grade 3) was observed in CCl4-induced fibrosis group while moderate inflammation (grade 2) was observed in SR group. Meanwhile the administration of IL-10 in group D resulted in a marked decrease in the grading of inflammation (grade 1) compared to CCl4-induced fibrosis group.No fibrosis was observed in the control group, however, a marked fibrosis reaching to cirrhosis (stages 3&4) was observed in CCl4-induced fibrosis group. The degree of fibrosis showed a mild decrease (stage 3) in SR group. Meanwhile the administration of IL-10 in group D resulted in a marked decrease in the stage of fibrosis to (stage 1).Serum TGF-β1 concentration dramatically increased in CCl4-induced fibrosis group compared to the control group. It also increased in SR group, but lesser than CCl4 treated group while it was significantly reduced in the IL-10 treated group. Conclusions:Our results provide evidence towards the potential effect of IL-10 as anti-TGF-β1 that could lead to a reduction of hepatic fibrosis.
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