Association between Type of Gene Polymorphisms of Interleukin 4 and Tumor Necrosis Factor Alpha and Chronicity of Immune Thrombocytopenia in Children

Document Type : Original Article

Authors

1 Department of Pediatrics, Faculty of Medicine, Port Said University, Port Said, Egypt

2 Department of Pediatrics, Faculty of Medicine, Suez Canal University, Ismailia Egypt

3 Department of Clinical Pathology, Faculty of Medicine, Suez Canal University, Ismailia Egypt

Abstract

Background: Immune thrombocytopenia (ITP) is an acquired autoimmune disease resulting from the production of antiplatelet antibodies. Interleukin 4 (IL4) is an important immunoregulatory cytokine. Also, pediatric ITP patients have excess levels of TNF-α (Tumor Necrosis Factor-alpha). Aim: detection of the frequency of IL4 Variable Number of Tandem Repeat (VNTR intron 3) and TNF-α gene (position -308) polymorphisms among Egyptian ITP children and to correlate these genes with the disease course. Patients and Methods: A case-control study was conducted on 50 Egyptian ITP patients and 50 age and sex-matched healthy controls, we excluded secondary ITP and patients with chronic inflammatory states. Interleukin 4 (VNTR intron 3) and TNF-α gene (position -308) gene polymorphisms were studied using polymerase chain reaction-restriction fragment length polymorphism assay (PCR-RFLP). Results: Most patients with acute ITP have a heterozygous variant of IL4 gene (75%), while 50% of chronic ITP patients have the wild variant of IL4. 43% of acute ITP have a mutant variant of the TNF gene and 50% of chronic ITP patients have a mutant variant of the TNF gene. Conclusion: TNF α gene polymorphism may have a role in ITP susceptibility in children. Also, TNF α and IL4 gene polymorphisms may affect the chronicity of thrombocytopenia.
 

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