Ismail, M., Fathy, H., Sahmoud, S., Hassan, A., Elfiky, S. (2023). Association between Type of Gene Polymorphisms of Interleukin 4 and Tumor Necrosis Factor Alpha and Chronicity of Immune Thrombocytopenia in Children. Suez Canal University Medical Journal, 26(7), 0-0. doi: 10.21608/scumj.2023.321377
Mona Ismail; Hesham Fathy; Shaimaa Sahmoud; Amany Hassan; Samar M. Elfiky. "Association between Type of Gene Polymorphisms of Interleukin 4 and Tumor Necrosis Factor Alpha and Chronicity of Immune Thrombocytopenia in Children". Suez Canal University Medical Journal, 26, 7, 2023, 0-0. doi: 10.21608/scumj.2023.321377
Ismail, M., Fathy, H., Sahmoud, S., Hassan, A., Elfiky, S. (2023). 'Association between Type of Gene Polymorphisms of Interleukin 4 and Tumor Necrosis Factor Alpha and Chronicity of Immune Thrombocytopenia in Children', Suez Canal University Medical Journal, 26(7), pp. 0-0. doi: 10.21608/scumj.2023.321377
Ismail, M., Fathy, H., Sahmoud, S., Hassan, A., Elfiky, S. Association between Type of Gene Polymorphisms of Interleukin 4 and Tumor Necrosis Factor Alpha and Chronicity of Immune Thrombocytopenia in Children. Suez Canal University Medical Journal, 2023; 26(7): 0-0. doi: 10.21608/scumj.2023.321377
Association between Type of Gene Polymorphisms of Interleukin 4 and Tumor Necrosis Factor Alpha and Chronicity of Immune Thrombocytopenia in Children
1Department of Pediatrics, Faculty of Medicine, Port Said University, Port Said, Egypt
2Department of Pediatrics, Faculty of Medicine, Suez Canal University, Ismailia Egypt
3Department of Clinical Pathology, Faculty of Medicine, Suez Canal University, Ismailia Egypt
Abstract
Background: Immune thrombocytopenia (ITP) is an acquired autoimmune disease resulting from the production of antiplatelet antibodies. Interleukin 4 (IL4) is an important immunoregulatory cytokine. Also, pediatric ITP patients have excess levels of TNF-α (Tumor Necrosis Factor-alpha). Aim:detection of the frequency of IL4 Variable Number of Tandem Repeat (VNTR intron 3) and TNF-α gene (position -308) polymorphisms among Egyptian ITP children and to correlate these genes with the disease course. Patients and Methods: A case-control study was conducted on 50 Egyptian ITP patients and 50 age and sex-matched healthy controls, we excluded secondary ITP and patients with chronic inflammatory states. Interleukin 4 (VNTR intron 3) and TNF-α gene (position -308) gene polymorphisms were studied using polymerase chain reaction-restriction fragment length polymorphism assay (PCR-RFLP). Results: Most patients with acute ITP have a heterozygous variant of IL4 gene (75%), while 50% of chronic ITP patients have the wild variant of IL4. 43% of acute ITP have a mutant variant of the TNF gene and 50% of chronic ITP patients have a mutant variant of the TNF gene. Conclusion: TNF α gene polymorphism may have a role in ITP susceptibility in children. Also, TNF α and IL4 gene polymorphisms may affect the chronicity of thrombocytopenia.
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