Tawfik, N., Abass, R., Abdallah, H., Gomaa, A. (2023). Assessment of Micro Ribonucleic Acid 320 Expression Level in Plasma of Vitiligo Patients Attending Suez Canal University Hospitals. Suez Canal University Medical Journal, 26(8), 0-0. doi: 10.21608/scumj.2023.322327
Noha Z. Tawfik; Rabab H. Abass; Hoda Y. Abdallah; Amal HA. Gomaa. "Assessment of Micro Ribonucleic Acid 320 Expression Level in Plasma of Vitiligo Patients Attending Suez Canal University Hospitals". Suez Canal University Medical Journal, 26, 8, 2023, 0-0. doi: 10.21608/scumj.2023.322327
Tawfik, N., Abass, R., Abdallah, H., Gomaa, A. (2023). 'Assessment of Micro Ribonucleic Acid 320 Expression Level in Plasma of Vitiligo Patients Attending Suez Canal University Hospitals', Suez Canal University Medical Journal, 26(8), pp. 0-0. doi: 10.21608/scumj.2023.322327
Tawfik, N., Abass, R., Abdallah, H., Gomaa, A. Assessment of Micro Ribonucleic Acid 320 Expression Level in Plasma of Vitiligo Patients Attending Suez Canal University Hospitals. Suez Canal University Medical Journal, 2023; 26(8): 0-0. doi: 10.21608/scumj.2023.322327
Assessment of Micro Ribonucleic Acid 320 Expression Level in Plasma of Vitiligo Patients Attending Suez Canal University Hospitals
1Department of Dermatology and Venereology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
2Department of Histology and Cell Biology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
Abstract
Background: Vitiligo is the most common skin disorder of depigmentation and is a multi-hypothesis disease requiring multifaceted interaction between autoimmune, biochemical, and genetic hypotheses. MiRNAs; are a major class of tiny noncoding RNAs. MiR-320 regulates inflammatory cytokines IL-2, IL-17, IFN-γ, and CXCL9 which are the main inflammatory cytokines contributing to the etiopathogenesis of vitiligo. Aim: To measure serum levels of miR-320 in vitiligo patients compared with controls to highlight the possible association of miR-320 with pathogenesis and severity of vitiligo. Subjects and Methods: A case-control study included 2 groups: group 1 involved 99 vitiligo patients; group 2 involved 99 healthy volunteers matched for age and sex. Disease activity and severity were assessed for each patient by VIDA and VASI scores. Serum miR-320 levels were assessed as blood samples were obtained from each patient and control than a quantitative measure of miRNA-320, using quantitative real-time PCR assay was done on all samples. Results: The mean level of miR-320 among patients was 23.05 ± 83.21; the mean level of miR-320 among controls was 1.0 ± 0.0. The variance between both groups was statistically significant (P < 0.05). Expression levels of miR-320 in vitiligo patients were higher than controls as a median of miR-320 levels in vitiligo patients was upregulated, being 3.19-fold higher than levels detected in controls. Conclusion: Serum miR-320 level was overexpressed in vitiligo patients compared to controls. Moreover, there was an insignificant association between miR-320 serum level and disease severity. Thus, miR-320 might have a key role in the etiopathogenesis of vitiligo.
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