Assessment of Micro Ribonucleic Acid 320 Expression Level in Plasma of ‎Vitiligo Patients Attending Suez Canal University Hospitals

Document Type : Original Article

Authors

1 Department of Dermatology and Venereology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.

2 Department of Histology and Cell Biology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.

Abstract

Background: Vitiligo is the most common skin disorder of depigmentation and is a multi-hypothesis disease requiring multifaceted interaction between ‎autoimmune‎, biochemical, and genetic hypotheses. MiRNAs; are a major ‎class of tiny noncoding RNAs. MiR-320 regulates inflammatory cytokines IL-2, IL-17, ‎IFN-γ, and CXCL9 which are the main inflammatory cytokines ‎contributing to the etiopathogenesis of vitiligo.‎ Aim: To measure serum levels of miR-320 in vitiligo‎‏ ‏patients compared with‎ controls to highlight the possible association of miR-320 with pathogenesis ‎and severity of vitiligo.‎ Subjects and Methods: A case-control study included 2 groups: group 1 involved 99 ‎vitiligo patients; group 2 involved 99 healthy volunteers matched for age ‎and sex. Disease activity and severity were assessed for each patient by VIDA ‎and VASI scores. Serum miR-320 levels were assessed as blood samples were ‎obtained from each patient and control than a quantitative measure of miRNA-320, ‎using ‎quantitative real-time PCR assay was done on all samples.‎ Results: The mean level of miR-320 among patients was 23.05 ± 83.21; the mean level ‎of miR-320 among controls was 1.0 ± 0.0. The variance between both groups was ‎statistically significant (P < 0.05). Expression levels of miR-320 in vitiligo ‎patients were higher than controls as a median of miR-320 levels ‎in vitiligo ‎patients was upregulated, being 3.19-fold higher than levels detected ‎in controls.‎ Conclusion: Serum miR-320 level was overexpressed in vitiligo patients ‎compared to controls. Moreover, there was an insignificant association ‎between miR-320 serum level and disease severity. Thus, miR-320 might have a ‎key role in the etiopathogenesis of vitiligo. ‎
 

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