Saad, N., Abdelkader, G., Salem, N., Ali, M., El-Fark, M. (2023). Mesenchymal Stem Cells Cultivation and Characterization from the Bone Marrow of Adult Male Albino Rats. Suez Canal University Medical Journal, 26(5), 12-17. doi: 10.21608/scumj.2023.306689
Nahla S. Saad; Ghada S. Abdelkader; Noha A. Salem; Mona H. Ali; Magdy M.O. El-Fark. "Mesenchymal Stem Cells Cultivation and Characterization from the Bone Marrow of Adult Male Albino Rats". Suez Canal University Medical Journal, 26, 5, 2023, 12-17. doi: 10.21608/scumj.2023.306689
Saad, N., Abdelkader, G., Salem, N., Ali, M., El-Fark, M. (2023). 'Mesenchymal Stem Cells Cultivation and Characterization from the Bone Marrow of Adult Male Albino Rats', Suez Canal University Medical Journal, 26(5), pp. 12-17. doi: 10.21608/scumj.2023.306689
Saad, N., Abdelkader, G., Salem, N., Ali, M., El-Fark, M. Mesenchymal Stem Cells Cultivation and Characterization from the Bone Marrow of Adult Male Albino Rats. Suez Canal University Medical Journal, 2023; 26(5): 12-17. doi: 10.21608/scumj.2023.306689
Mesenchymal Stem Cells Cultivation and Characterization from the Bone Marrow of Adult Male Albino Rats
Department of Human Anatomy and Embryology, Faculty of Medicine, Suez Canal University, Egypt
Abstract
Background: Stem cells are self-renewing, undifferentiated cells that can be differentiated into functional cells. Mesenchymal stem cells (MSCs) are a subpopulation of mesodermal stem cells population that can be found in numerous living tissues along with bone marrow. Numerous studies have begun to reveal the characteristics of MSCs, which go far beyond forming connective tissue and their simple derivation from various tissues, making them attractive for research. Aim: The present study aimed to isolate, culture, and characterize the MSCs from the bone marrow of long bones of adult male albino rats. Materials and Methods: both femoral bones and tibia were obtained from adult male albino rats. Their bone marrow was washed using complete media formed of DMEM + 10% FPS+ 1% Pen/strept. The mixture was seeded in the culture flask and incubated at 370 humidified atmosphere containing a 5% co2 incubator. The culture flasks were examined under a light microscope to monitor the growth of cells. The cells were then harvested and subcultured using the same technique. Subcultured cells were identified using CD 34 & 44 surface markers. Results: the subcultured MSCs appeared as a sheet of adherent cells having a polygonal appearance with prominent nucleolus & a well-defined cell process. Most of the cells showed positive expression of the CD44 surface marker and negative expression of CD 34. Conclusions: cells that were isolated and cultured from the bone marrow of adult male albino rats had the morphological & immunophenotyping characteristics of MSCs.
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