Experimental Study for Preparation and Characterization of a Mucoadhesive Gel as a Vehicle for Intranasal Delivery of Topical Antibiotic Therapy

Document Type : Original Article

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Department of Otorhinolaryngology, Faculty of Medicine, Suez Canal University, Egypt

Abstract

Background: Mucoadhesive nasal gel provides a good platform for drug delivery to the nasal mucosa. We aimedto detect the histologic effect of mucoadhesive-loaded antibiotics on the nasal mucosa of the rabbits by light microscope and scanning electron microscope to detect the ultra-structure changes.Materials and Methods:A total of 36 adult male rabbits were divided into 3 groups. Vehicle control group (A): Non-diseased, receives intranasal mucoadhesive gel. Group (B): Non-diseased, receives intranasal Neomycin-loaded mucoadhesive gel.Group (C): Non-diseased, receives intranasal ciprofloxacin-loaded mucoadhesive gel.Part of the nasal mucosa was stained by Toluidine blue for scanning light microscopy.The other part of the Nasal mucosa was immediately fixed in 1.5% glutaraldehyde in phosphate-buffered saline (pH 7.4) for 2 h at room temperature, and determination of morphological properties and microscopic ultra-structure was performed. Results:There is a statistically insignificant difference in histological changes in inflammatory cells of the Ciprofloxacin group also surface mucosa shows deciliation of a wide area of the epithelial cells andnasal mucosa shows necrobiosis of numerous epithelial cells. Congestion is significantly higher in the Neomycin group (46.1%) than in the Ciprofloxacin group (7.7%). Conclusion: Antibiotic-loaded mucoadhesive gel showed minimal damage with preserved integrity of the nasal mucosa, so it could be considered a safe vehicle for delivering topical antibiotics.
 

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