Assessment of Chemokine Motif Ligand 4 Gene Polymorphism in Rheumatoid Arthritis Patients and its Correlation with Disease Activity

Elshamy, Fatma; Omar, Hanan; El-Shahaly, Mohsen; Fathy, Amal

doi:10.21608/scumj.2023.292664

Assessment of Chemokine Motif Ligand 4 Gene Polymorphism in Rheumatoid Arthritis Patients and its Correlation with Disease Activity

Document Type : Original Article

Authors

¹ Department of Clinical Pathology, Faculty of Medicine, Suez Canal University, Egypt

² Department of Physical Medicine and Rehabilitation, Faculty of Medicine, Suez Canal University, Egypt

10.21608/scumj.2023.292664

Abstract

Background The recent development of biological-based antirheumatic therapies that target inflammatory pathways in RA has enabled increasing numbers of patients to achieve very low levels of disease activity, yet a substantial proportion of RA patients remain treatment-refractory. CCL4 SNP is suspected to be one of these aberrations as it has been related to other diseases yet its role in RA is not well studied. Aim of work: this study aimed to assess the association between the chemokine motif ligand 4 (CCL4) Gene Polymorphism and susceptibility to RA disease and its correlation to the activity of the disease. Subjects and Methods this is a case-control study where CCL4 polymorphisms (SNPs1719153) genotyping and its relation to RA activity were assessed by a real-time PCR in 50 RA patients in comparison with age and sex-matched healthy control. Results: The results of the present study showed a non-statistically significant difference between the patients and controls in the distribution of the genotypes (AA/AT) and alleles (A/T) frequency. And no significant differences between these genotypes in terms of medication use, clinical status, or disease activity. Conclusion: CCL4 gene SNP rs1719153 genotypes (AA and AT) as well as CCL4 SNP rs1719153 alleles (A and T) did not significantly differ between RA patients and normal controls and had no relation to disease activity. To the best of our knowledge, this study is one of few studies to identify the distribution of rs1719153 SNPs in RA in the Egyptian population.

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