Awad, M., Abd-Ellatif, R., Ibrahim, S., Abd Elmaaboud, M., El-Shaer, R. (2023). Role of Heme Oxygenase (HO)-1 Enzyme in the Protective and Therapeutic Effect of Omega 3 Fatty Acids on Cisplatin-induced Hepatic and Renal Toxicity in Rats. Suez Canal University Medical Journal, 26(1), 17-39. doi: 10.21608/scumj.2023.283960
Marwa M. Awad; Rania N. Abd-Ellatif; Sarah Ibrahim; Maaly A. Abd Elmaaboud; Rehab A.A. El-Shaer. "Role of Heme Oxygenase (HO)-1 Enzyme in the Protective and Therapeutic Effect of Omega 3 Fatty Acids on Cisplatin-induced Hepatic and Renal Toxicity in Rats". Suez Canal University Medical Journal, 26, 1, 2023, 17-39. doi: 10.21608/scumj.2023.283960
Awad, M., Abd-Ellatif, R., Ibrahim, S., Abd Elmaaboud, M., El-Shaer, R. (2023). 'Role of Heme Oxygenase (HO)-1 Enzyme in the Protective and Therapeutic Effect of Omega 3 Fatty Acids on Cisplatin-induced Hepatic and Renal Toxicity in Rats', Suez Canal University Medical Journal, 26(1), pp. 17-39. doi: 10.21608/scumj.2023.283960
Awad, M., Abd-Ellatif, R., Ibrahim, S., Abd Elmaaboud, M., El-Shaer, R. Role of Heme Oxygenase (HO)-1 Enzyme in the Protective and Therapeutic Effect of Omega 3 Fatty Acids on Cisplatin-induced Hepatic and Renal Toxicity in Rats. Suez Canal University Medical Journal, 2023; 26(1): 17-39. doi: 10.21608/scumj.2023.283960
Role of Heme Oxygenase (HO)-1 Enzyme in the Protective and Therapeutic Effect of Omega 3 Fatty Acids on Cisplatin-induced Hepatic and Renal Toxicity in Rats
1Physiology Department, Faculty of Medicine, Tanta University, Egypt.
2Biochemistry Department, Faculty of Medicine, Tanta University, Egypt.
3Anatomy Department, Faculty of Medicine, Tanta University, Egypt.
4Pharmacology Department, Faculty of Medicine, Tanta University, Egypt
Abstract
Background: Cisplatin is frequently used as an anticancer medication. Nephrotoxicity and hepatotoxicity are triggered by its usage, causing patients to limit their long-term therapy. Aim: To investigate the underlying mechanism of omega-3 (fatty acids) effect on hepatorenal toxicity induced by cisplatin in rats and to detect whether it has a protective or therapeutic effect or both and the role of HO-1 enzyme in both effects. Materials and Methods: 40 male rats were divided into four equal groups: Control group: received i.p. saline+ corn oil orally, Cisplatin Group: received i.p. CP (12 mg/kg)+ corn oil orally, Cisplatin+ ω-3 pretreatment group: received i.p. CP following 10 days of ω-3 pretreatment in dose {(270 mg/kg) EPA, (180 mg/kg)} and Cisplatin+ ω-3 post-treatment group: received i.p. CP followed by 10 days of ω-3 post-treatment in dose as the previous group. Liver and kidney function, serum (HO-1), serum& tissue (TNF-α, IL-10), tissue (NFkB, GSH, MDA), and NrF2 gene expressions were measured. Results: Cisplatin-induced marked hepatorenal failure; detected by elevation of serum: AST, ALT, creatinine, and urea. Also, serum& tissue (TNF-α, IL-10), and tissue (NFkB, GSH, MDA) were significantly changed with no change in NrF2 gene expressions as compared to the control. On other hand, pre or post-ω-3 intake significantly corrected the changed markers. Liver and renal histopathological and immunohistochemical changes confirmed the biochemical results in all groups. Conclusion: Cisplatin treatment impairs liver and kidney function, while ω-3 supplementation could avoid this toxicity, with the protective response appearing to be more beneficial than the therapeutic effect.
Top