Suez Canal University, Faculty of MedicineSuez Canal University Medical Journal1110-699926120230101A Study of Plasma miR-23a Expression in Vitiligo Patients11028395810.21608/scumj.2023.283958ENDoaa O.SalmanMedical Genetics Unit, Department of Histology & Cell Biology, Faculty of Medicine, Suez Canal University, EgyptEman A.MohammedMedical Genetics Unit, Department of Histology & Cell Biology, Faculty of Medicine, Suez Canal University, EgyptEman A.ToraihMedical Genetics Unit, Department of Histology & Cell Biology, Faculty of Medicine, Suez Canal University, EgyptAlia EllawindyMedical Genetics Unit, Department of Histology & Cell Biology, Faculty of Medicine, Suez Canal University, EgyptJournal Article20230202<strong><em>Background:</em></strong><strong> Vitiligo is a chronic autoimmune skin depigmentation disorder with multifactorial causation, involving genetic susceptibility, immunological events, and environmental triggers. The exact molecular mechanisms of vitiligo development and progression are poorly understood. Recent studies reported microRNAs as promising biomarkers for disease detection and molecular targets for future treatment. <em>Aim:</em> Evaluating the expression level of circulating miR-23a in vitiligo patients and its association with the clinical features of vitiligo. <em>Subjects and Methods</em>: This is a case-control study comprising 50 vitiligo patients and 44 healthy controls. Plasma miR-23a expression levels were estimated by quantitative real-time PCR. Bioinformatic analysis for the miR-23a gene was performed. <em>Results:</em> Vitiligo patients displayed significantly lower circulating miR-23a expression levels compared to healthy controls. There was a significant negative correlation between miR-23a fold change and </strong><strong>Vitiligo Area Severity Index </strong><strong>(<em>p < /em>= 0.003). Plasma miR-23a levels discriminated between vitiligo patients and controls with 60 % specificity and 64% sensitivity at the optimal cut-off value of 0.23 and likelihood ratio 1.61 (AUC=0.67). <em>Conclusion</em>: miR-23a along with its putative target genes could play a role in vitiligo pathogenesis.</em></strong><br /> https://scumj.journals.ekb.eg/article_283958_6d46975ec27f1a2f92643774c8b7f573.pdfSuez Canal University, Faculty of MedicineSuez Canal University Medical Journal1110-699926120230101Effects of the Nano-Profenofos Pesticide on the Chromosomes of Albino Rats111628395910.21608/scumj.2023.283959ENAsma S.HarfoshPlant Protection Research Institute, Agriculture Research Center, Dokki, Giza, Egypt.Ahmed H.Abo-GhaliaSuez Canal University, Faculty of Science, Zoology department, Ismailia, EgyptAhmed M.HegabPlant Protection Research Institute, Agriculture Research Center, Dokki, Giza, Egypt.Mohamed I.El-BakhshwangiPlant Protection Research Institute, Agriculture Research Center, Dokki, Giza, Egypt.Howyda I.Abdel-HalimSuez Canal University, Faculty of Science, Zoology department, Ismailia, EgyptJournal Article20230202<strong><em>Background:</em></strong><strong>The management of insect pests through the formulations of nanomaterials-based insecticides is not uncommon recently. Traditional strategies used in agriculture are insufficient, and the application of chemical pesticides has adverse effects on animals and human beings apart from the decline in soil fertility. Therefore, nanotechnology would provide green and efficient alternatives for the management of insect pests in agriculture without harming nature. However, the disadvantages of nanotechnology are possible in both occupational and residential environments. </strong><strong><em>Aim:</em></strong><strong> This study aimed at the evaluation of the genotoxic effect of the pesticide Profenofos (organophosphorus) and its nanoparticles in rats. This was assessed by the study of chromosomal aberrations</strong><strong>. <em>Materials and Methods</em>: </strong><strong>Healthy male rats were orally fed with different doses (1/20 and 1/40 of the LD<sub>50</sub>) of Profenofos and nano-Profenofos in multi doses for three successive days at two different time intervals (24 hours and one week) post-treatment. <em>Results:</em> The data revealed a significant increase in the number of chromosomal abnormalities in both pesticide-treated animals compared to their controls. The maximum genotoxic effect was induced by the nano-Profenofos pesticide. </strong><strong><em>Conclusion:</em></strong><strong> The results obtained in this study suggest that nano-pesticides may have higher genotoxic and biological effects than their regular pesticides.</strong>
https://scumj.journals.ekb.eg/article_283959_e1a6b5dd6c85f800cb1f127b48ff73ac.pdfSuez Canal University, Faculty of MedicineSuez Canal University Medical Journal1110-699926120230101Role of Heme Oxygenase (HO)-1 Enzyme in the Protective and Therapeutic Effect of Omega 3 Fatty Acids on Cisplatin-induced Hepatic and Renal Toxicity in Rats173928396010.21608/scumj.2023.283960ENMarwa M.AwadPhysiology Department, Faculty of Medicine, Tanta University, Egypt.Rania N.Abd-EllatifBiochemistry Department, Faculty of Medicine, Tanta University, Egypt.Sarah IbrahimAnatomy Department, Faculty of Medicine, Tanta University, Egypt.Maaly A.Abd ElmaaboudPharmacology Department, Faculty of Medicine, Tanta University, EgyptRehab A.A.El-ShaerPhysiology Department, Faculty of Medicine, Tanta University, Egypt.Journal Article20230202<strong><em>Background:</em></strong><strong> Cisplatin is frequently used as an anticancer medication. Nephrotoxicity and hepatotoxicity are triggered by its usage, causing patients to limit their long-term therapy. <em>Aim:</em> To investigate the underlying mechanism of omega-3 (fatty acids) effect on hepatorenal toxicity induced by cisplatin in rats and to detect whether it has a protective or therapeutic effect or both and the role of HO-1 enzyme in both effects. <em>Materials and Methods:</em> 40 male rats were divided into four equal groups: Control group: received i.p. saline+ corn oil orally, Cisplatin Group: received i.p. CP (12 mg/kg)+ corn oil orally, Cisplatin+ ω-3 pretreatment group: received i.p. CP following 10 days of ω-3 pretreatment in dose {(270 mg/kg) EPA, (180 mg/kg)} and Cisplatin+ ω-3 post-treatment group: received i.p. CP followed by 10 days of ω-3 post-treatment in dose as the previous group. Liver and kidney function, serum (HO-1), serum& tissue (TNF-α, IL-10), tissue (NFkB, GSH, MDA), and NrF2 gene expressions were measured. <em>Results:</em> Cisplatin-induced marked hepatorenal failure; detected by elevation of serum: AST, ALT, creatinine, and urea. Also, serum& tissue (TNF-α, IL-10), and tissue (NFkB, GSH, MDA) were significantly changed with no change in NrF2 gene expressions as compared to the control. On other hand, pre or post-ω-3 intake significantly corrected the changed markers. Liver and renal histopathological and immunohistochemical changes confirmed the biochemical results in all groups. <em>Conclusion:</em> Cisplatin treatment impairs liver and kidney function, while ω-3 supplementation could avoid this toxicity, with the protective response appearing to be more beneficial than the therapeutic effect.</strong><br /> https://scumj.journals.ekb.eg/article_283960_c4d785e8e2f5bd9178cb68734a4a7d12.pdfSuez Canal University, Faculty of MedicineSuez Canal University Medical Journal1110-699926120230101Role of Exercise Stress Echocardiography in the Detection of Subclinical Diastolic Dysfunction in Asymptomatic Diabetics404628400010.21608/scumj.2023.284000ENAhmed M.FareedCardiology Department, Faculty of Medicine, Suez Canal University, EgyptFathy A.NadaCardiology Department, Faculty of Medicine, Suez Canal University, EgyptAhmed A.ElhawaryCardiology Department, Faculty of Medicine, Suez Canal University, EgyptMohamed A.OrabyCardiology Department, Faculty of Medicine, Suez Canal University, EgyptAhmed H.AbodelmonemCardiology Department, Faculty of Medicine, Suez Canal University, EgyptJournal Article20230203<strong><em>Background:</em></strong><strong>Subclinical left ventricular dysfunction is not uncommon and is a</strong><strong> predictor of heart failure. Diabetes commonly presents with exertional dyspnea and reduced exercise tolerance, which is due to diastolic dysfunction</strong><strong>.</strong><strong> Diastolic exercise echocardiography is a relatively novel modality to detect latent diastolic dysfunction.</strong><strong> <em>Aim:</em></strong><strong> We </strong><strong>aimed to evaluate the different parameters that affect raised filling pressure during exercise in asymptomatic diabetics using exercise stress echocardiography.</strong><strong> <em>Subjects and Methods:</em></strong><strong>This is a case-control study that included 70 persons (35 diabetics and 35 normal volunteers age and sex-matched) who had exertional dyspnea but were asymptomatic at rest. They had a normal systolic and diastolic functions at rest. They did treadmill exercise stress ECG. Post-peak stress images were obtained as quickly as possible after the patient transferred from the treadmill to the imaging table measuring the E/e' ratio. </strong><strong><em>Results:</em></strong><strong>Filling pressure raised dramatically in the diabetic group compared to non-diabetics. Using linear regression analysis, HbA1c as a continuous variable, duration of DM, and waist circumference showed a positive linear significant association with </strong><strong>E/e’ </strong><strong>during exercise. </strong><strong><em>Conclusion:</em></strong><strong>HbA1c level, duration of DM, waist circumference, and eGFR </strong><strong>are related to subclinical diastolic dysfunction in asymptomatic diabetics during exercise.</strong>
https://scumj.journals.ekb.eg/article_284000_5f2e3848e3b5765afd67d44862c7ac19.pdfSuez Canal University, Faculty of MedicineSuez Canal University Medical Journal1110-699926120230101Tissue Expression of Protease Activated Receptor 2 in Patients with Pruritus in Chronic Kidney Disease475428400110.21608/scumj.2023.284001ENHesham A.NadaDepartment of Dermatology, Venereology and Andrology, Faculty of Medicine, Suez Canal University, Egypt.0000 0001 7094 5462Gamal A.HafezDepartment of Pathology, Faculty of Medicine, Suez Canal University, Egypt.Mohammed M.KeshawyDepartment of Internal Medicine; Nephrology division, Faculty of Medicine, Suez Canal University, EgyptEman G.AbdelrahmanDepartment of Dermatology, Venereology and Andrology, Faculty of Medicine, Suez Canal University, Egypt.Journal Article20230203<strong><em>Background</em></strong><strong>: Uremic pruritus has been defined as itching that is directly related to kidney disease, without any other comorbid condition that causes itching. </strong><strong>The exact pathogenesis of uremic pruritus remains unclear but, protease</strong><strong>-activated receptor 2 (PAR2) is claimed to have a pivotal role in the pathogenesis of uremic pruritus.<em> Aim</em>: Evaluation of PAR2 activity in the epidermis of patients with chronic kidney disease (CKD) complaining of pruritus. <em>Subjects and Methods:</em> Case-control study on 48 individuals (24 CKD patients complaining from pruritus and 24 matched healthy controls).</strong><strong> Skin biopsy specimens from all participants were taken from a similar area of the back of forearms then i</strong><strong>mmunohistochemical analysis for PAR2 activity in the epidermis was performed</strong><strong>. </strong><strong><em>Results:</em></strong><strong> Data revealed that epidermal PAR-2 expression was significantly higher in CKD patients compared to healthy controls. PAR2 staining intensity in skin biopsies was moderate in 9 (37.5%) patients and strong in 15 (62.5%) patients in the cases group. On the other hand, PAR2 staining was moderate in all 24 (100%) cases in the control group which was statistically significant.</strong><strong><em>Conclusion:</em></strong><strong> Epidermal PAR-2 expression is increased in CKD patients with pruritus.</strong>https://scumj.journals.ekb.eg/article_284001_eb9e422acee69b66333122b78bbb5ca9.pdfSuez Canal University, Faculty of MedicineSuez Canal University Medical Journal1110-699926120230101Comparison of Endovenous Laser versus Radiofrequency Ablation in the Treatment of Primary Long Saphenous Varicose Veins556028423510.21608/scumj.2023.284235ENShimaa A.MubarakVascular and Endovascular Unit, Department of Surgery, Suez Canal University Hospital, Ismailia, Egypt.Sherif A.ReffatVascular and Endovascular Unit, Department of Surgery, Suez Canal University Hospital, Ismailia, Egypt.Meera R.BoulosVascular and Endovascular Unit, Department of Surgery, Suez Canal University Hospital, Ismailia, Egypt.Journal Article20230206<strong><em>Background: </em></strong><strong>Endovenous laser ablation (EVLA)and radiofrequency ablation(RFA)are both associated with excellent technical, clinical, and patient-reported outcomes for the treatment of varicose veins.<em> Aim</em>: to compare endovenous laser ablation and radiofrequency ablation in the treatment of primary long saphenous varicose veins.<em> Patients and Method</em>:</strong><strong> 40 patients scheduled for treatment of varicose veins were included and were divided randomly into 2 equal groups. Endovenous laser ablation was used for the first group and compared to a second group treated with Radiofrequency ablation. The success rate and postoperative results of both groups were compared to each other. <em>Results: </em>closure rate in EVLA group was 100% and in RFA group was 80%, radiofrequency ablation, and EVLA complications: skin burn 5% and 0 %, hyperpigmentation 10% and 0 %, and groin hematoma 0% and 5% respectively. <em>Conclusion</em>: EVLA procedure seems to be superior to RFA in the treatment of long saphenous varicose veins.</strong><br /> https://scumj.journals.ekb.eg/article_284235_d81c92ea311d103c72123f12c2b4c411.pdfSuez Canal University, Faculty of MedicineSuez Canal University Medical Journal1110-699926120230101Tissue Expression of Nuclear Factor Kappa Beta in Psoriasis Vulgaris Before and After Psoralen Ultraviolet-A Therapy: A Prospective Study616928423610.21608/scumj.2023.284236ENHesham NadaDepartment of Dermatology and Venereology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt0000 0001 7094 5462Sherine El-SamanDepartment of Dermatology and Venereology, Faculty of Medicine, Suez Canal University, Ismailia, EgyptAbeer MohamedDepartment of Histology, Faculty of Medicine, Suez Canal University, Ismailia, EgyptJournal Article20230206<strong><em>Background:</em></strong><strong>Nuclear factor kappa beta is an inducible nuclear transcription factor regulating a range of cellular processes. Nuclear factor kappa beta </strong><strong>is considered to impact many cellular phenomena such as inflammation, immune responses, proliferation, differentiation, apoptosis, and tumor progression, therefore considered to have a major role in many inflammatory skin diseases including the pathogenesis of</strong><strong> psoriasis. <em>Aim:</em> This study aims to investigate n</strong><strong>uclear factor kappa beta </strong><strong>tissue expression </strong><strong>in patients with psoriasis vulgaris before and after Psoralen Ultraviolet A therapy. </strong><strong><em>Subjects and Methods:</em></strong><strong> Thirty patients with generalized chronic plaque psoriasis were assigned as a test group (Group I) and 10 age-and sex-matched healthy individuals assigned as a control group (Group II) were enrolled in this study. Skin biopsy specimens were subjected to histopathological study and immunohistochemical determination of</strong><strong>n</strong><strong>uclear factor kappa beta before and after 30 sessions of Psoralen Ultraviolet A therapy. </strong><strong><em>Results:</em></strong><strong> This study revealed highly constitutive tissue expression of</strong><strong>n</strong><strong>uclear factor kappa beta in all psoriatic lesions with variable degrees in comparison with control specimens </strong><strong>(p < 0.001). Also, there was a significant decrease in </strong><strong>n</strong><strong>uclear factor kappa beta </strong><strong>expression</strong><strong>in psoriatic skin after </strong><strong>Psoralen Ultraviolet A</strong><strong> therapy (p < 0.001).</strong><strong><em>Conclusion:</em></strong><strong>Psoralen Ultraviolet A</strong><strong> therapy among psoriatic patients demonstrated a decrease in PASI score, epidermal thickness; mononuclear cellular infiltration, as well as</strong><strong> nucleocytoplasmic positivity (active </strong><strong>n</strong><strong>uclear factor kappa beta </strong><strong>form) </strong><strong>expression, accordingly</strong><strong> selective blockade of </strong><strong>n</strong><strong>uclear factor kappa beta </strong><strong>could be an effective treatment for psoriasis</strong><strong>.</strong><br /> https://scumj.journals.ekb.eg/article_284236_e416a91d08423362ebf27975b38fdc31.pdfSuez Canal University, Faculty of MedicineSuez Canal University Medical Journal1110-699926120230101Evaluation of Sentinel Lymph Node Mapping in Uterine Cancer Staging707528728410.21608/scumj.2023.287284ENHamada FathyDepartment of Surgery, Faculty of Medicine, Suez Canal University, Ismailia, EgyptMahmoud HussinDepartment of Surgery, Faculty of Medicine, Suez Canal University, Ismailia, EgyptAhmed AboBakrDepartment of Surgery, Faculty of Medicine, Suez Canal University, Ismailia, EgyptSolimam El-KammashDepartment of Surgery, Faculty of Medicine, Suez Canal University, Ismailia, EgyptAhmed M.GomaaDepartment of Surgery, Faculty of Medicine, Suez Canal University, Ismailia, EgyptJournal Article20230225<strong><em>Background: </em></strong><strong>Lymphatic mapping recently emerged as a promising new strategy and is increasingly being adopted by gynecologic oncology practices worldwide. The sentinel lymph node is a technique used to properly detect nodal metastases hence improving staging accuracy with the subsequent proper application of adjuvant therapy. <em>Aim:</em> to evaluate of the accuracy of sentinel lymph node biopsy using a patant blue dye in endometrial and cervical malignancies. <em>Patients and Methods:</em> Twenty-six patients, </strong><strong>diagnosed with endometrial cancers stage (I/II) by MRI,</strong><strong> and three patients, </strong><strong>diagnosed with cervical cancers stage (Ia/IIa) by MRI,</strong><strong> were enrolled in the study. <em>Results: </em>Sentinel lymph node(s) were successfully identified in 18 patients (69.2%) with endometrial carcinoma and one case with cancer cervix. The sensitivity of sentinel Lymph node mapping in the detection of lymph node metastasis was 100% with a negative predictive value of 100% and 0% false negative rate</strong><strong>. </strong><strong>There was a statistically significant inverse relation between sentinel lymph node detection and tumor grading. There was a statistically significant relation between sentinel lymph node detection and both cervical involvement and positive lymph node metastasis. <em>Conclusion: </em>Sentinel Lymph node mapping in cases of early-stage endometrial cancer is a good option to avoid overtreatment with unnecessary removal of more lymph nodes, which may result in an increase in perioperative adverse effects.</strong><br /> https://scumj.journals.ekb.eg/article_287284_d2f9858feb769a35c7e899f47725d652.pdfSuez Canal University, Faculty of MedicineSuez Canal University Medical Journal1110-699926120230101Validity of CA-125 in the Diagnosis of Absent Lymph Node Metastasis in Endometrial Cancer Patients768128730110.21608/scumj.2023.287301ENAhmed M.GomaaDepartment of Surgery, Faculty of Medicine, Suez Canal University, Ismailia, EgyptMahmoud G.HussinDepartment of Surgery, Faculty of Medicine, Suez Canal University, Ismailia, EgyptAhmed AboBakrDepartment of Surgery, Faculty of Medicine, Suez Canal University, Ismailia, EgyptSolimam El-KammashDepartment of Surgery, Faculty of Medicine, Suez Canal University, Ismailia, EgyptHamada FathyDepartment of Surgery, Faculty of Medicine, Suez Canal University, Ismailia, EgyptJournal Article20230225<strong><em>Background:</em></strong>Serum CA 125 is elevated in many physiological and pathological conditions that are related to endometrial proliferation as menstrual cycle, pregnancy, endometriosis, and endometrial carcinoma. Many researchers studied Serum CA 125 level and its role in the assessment of endometrial cancer patients. The relation between the CA 125 level and many variables was demonstrated, such as lymph node involvement, myometrial invasion, disease stage, and overall survival. <strong><em>Aim</em></strong>: to evaluate the relation between the tumor marker CA 125 and lymph node metastasis. <strong><em>Patients and Methods:</em> </strong>A total number of twenty-six patients with endometrial carcinoma were enrolled in the study. Serum CA 125 was withdrawn preoperatively to all patients. The patients were subjected to total abdominal hysterectomy and bilateral salpingo-oophorectomy. A method of lymphatic mapping using patent blue dye was performed. The sentinel lymph node(s) and non-sentinel lymph nodes were sent for H&E staining. <strong><em>Results: </em></strong>On evaluating serum CA-125 of ≤35 IU/mL in predicting the absence of lymph node involvement as confirmed pathologically, the sensitivity was 87%, specificity 100%, positive predictive value 100%, negative predictive value 50% with accuracy 88.5%. <strong><em>Conclusion:</em></strong>CA 125 is a valid method in the preoperative assessment of patients with endometrial carcinoma.<br /> https://scumj.journals.ekb.eg/article_287301_286fa9fb63f0d659c7d09979f687a7e2.pdfSuez Canal University, Faculty of MedicineSuez Canal University Medical Journal1110-699926120230101Impact of Degeneration of the Prelimbic and Infralimbic Cortices on Psychomotor Activity and Cognitive Func-tion in Rats Stereotaxically Injected with Ibotenate829128754810.21608/scumj.2023.287548ENMeran MostafaHuman Physiology Department, Faculty of Medicine, Suez Canal University.Mona MansourHuman Physiology Department, Faculty of Medicine, Suez Canal University.Hani S.HafezZoology Department, Faculty of Science, Suez University, Egypt.Faten AbbasHuman Physiology Department, Faculty of Medicine, Suez Canal University.Journal Article20230226<strong><em>Background:</em></strong><strong> Little is understood about how mild cognitive impairment affects motor function (MCI). Memory and psychomotor functions are assumed to be regulated by the medial prefrontal cortex (mPFC). The mPFC consists of a total of three distinct subareas. Thus, the purpose of this work was to specifically damage the prelimbic (PrL) and infralimbic (IFL) subareas utilizing a rodent stereotaxis injection of a relatively low dose of ibotenate. <em>Aim:</em> to investigate the impact of the PrL and IFL subareas lesions on psychomotor activities and memory among stereotaxically injected rats with ibotenate. <em>Materials and Methods:</em> For this study, a total of 36 white albino rats were employed. Three groups were designed: the normal control group (GI), the bilateral sham group (GII) which included injected rats with phosphate-buffered saline (PBS) in the PrL and IL subareas, and the bilateral lesioned group (GIII) which included injected rats with ibotenate in the PrL and IL cortices. Then rats were undergone through the open field test (OFT) and hole board test (HB). Transcardial perfusion and brain extraction for tissue preparation and histological evaluation were done at the end of the experiment. <em>Results:</em> the group with bilateral PrL and IFL lesions had intact psychomotor functions and spatial reference memory, but they started to lose short-term memory. <em>Conclusions:</em> PrL and IFL subareas of the mPFC degeneration don’t impair psychomotor functions or spatial reference memory but can impact short-term memory in rats.</strong><br /> <strong><em>Keywords:</em></strong>mild cognitive impairment, medial prefrontal cortex, rodent stereotaxis, ibotenatehttps://scumj.journals.ekb.eg/article_287548_feeb90ea2a31a3a0fe2bef8e22f0b83a.pdfSuez Canal University, Faculty of MedicineSuez Canal University Medical Journal1110-699926120230101Assessment of Chemokine Motif Ligand 4 Gene Polymorphism in Rheumatoid Arthritis Patients and its Correlation with Disease Activity929929266410.21608/scumj.2023.292664ENFatma ElshamyDepartment of Clinical Pathology, Faculty of Medicine, Suez Canal University, EgyptHanan OmarDepartment of Clinical Pathology, Faculty of Medicine, Suez Canal University, EgyptMohsen El-ShahalyDepartment of Physical Medicine and Rehabilitation, Faculty of Medicine, Suez Canal University, EgyptAmal FathyDepartment of Clinical Pathology, Faculty of Medicine, Suez Canal University, EgyptJournal Article20230329<strong><em>Background</em></strong><strong> The recent development of biological-based antirheumatic therapies that target inflammatory pathways in RA has enabled increasing numbers of patients to achieve very low levels of disease activity, yet a substantial proportion of RA patients remain treatment-refractory. CCL4 SNP is suspected to be one of these aberrations as it has been related to other diseases yet its role in RA is not well studied. <em>Aim of work:</em> this study aimed to assess the association between the chemokine motif ligand 4 (CCL4) Gene Polymorphism and susceptibility to RA disease and its correlation to the activity of the disease. <em>Subjects and Methods</em> this is a case-control study where CCL4 polymorphisms (SNPs1719153) genotyping and its relation to RA activity were assessed by a real-time PCR in 50 RA patients in comparison with age and sex-matched healthy control. <em>Results:</em> The results of the present study showed a non-statistically significant difference between the patients and controls in the distribution of the genotypes (AA/AT) and alleles (A/T) frequency. And no significant differences between these genotypes in terms of medication use, clinical status, or disease activity<em>. Conclusion:</em> </strong><strong>CCL4 gene SNP </strong><strong>rs1719153</strong><strong> genotypes (AA and AT) as well as CCL4 SNP rs1719153 alleles (A and T) did not significantly differ between RA patients and normal controls and had no relation to disease activity. </strong><strong>To the best of our knowledge, this study is one of few studies to identify the distribution of rs1719153 SNPs in RA in the Egyptian population. </strong>https://scumj.journals.ekb.eg/article_292664_c0d4c18b83c8be996dba32684a3b5750.pdfSuez Canal University, Faculty of MedicineSuez Canal University Medical Journal1110-699926120230101Assessment of the Frequency of the G6PD Mediterranean Gene Mutation 563 C→T (rs5030868) in Children attending Suez Canal University Hospital10010634246610.21608/scumj.2023.342466ENAhmed A. ElhadidyDepartment of Clinical Pathology, Faculty of Medicine, Suez Canal University, EgyptAlaa ELdin S. AbdelhamidDepartment of Clinical Pathology, Faculty of Medicine, Suez Canal University, EgyptFadia M. AttiaDepartment of Clinical Pathology, Faculty of Medicine, Suez Canal University, EgyptGehan IbrahimDepartment of Clinical Pathology, Faculty of Medicine, Suez Canal University, EgyptMarwa A. IbrahimDepartment of Pediatrics, Faculty of Medicine, Suez Canal University, EgyptJournal Article20240221<strong><em>Background:</em></strong><strong>Glucose-6-phosphate dehydrogenase (G6PD) deficiency is known to be the most common enzymopathy in the world affecting ~7% of the global population. The G6PDgene, located in the long arm of chromosome X (Xq28), consists of 13 exons and 12 introns encoding 515 amino acids. Over 200 G6PDmutations have been reported worldwide, where 140 mutations or combined mutations are found to be hematologically important. <em>Aim:</em></strong><strong>To assess the frequency of the G6PD </strong><strong>Mediterranean (Med) gene mutation 563 C</strong><strong>→</strong><strong>T </strong><strong>in children attending Suez Canal University Hospital and its relation to G6PD silent gene mutation 1311</strong> <strong>C</strong><strong>→</strong><strong>T. <em>Subjects and Methods:</em> This study was conducted on 50 G6PD deficiency patients who were previously diagnosed and confirmed by quantification of G6PD enzyme activity, 44 of them were males while 6 patients were females. The control group was 20 apparently healthy children with matched age and sex with the patients' group. Each of the study groups was tested for the following: complete blood count, G6PD enzyme activity quantification, and testing the presence or absence of the</strong><strong> G6PD </strong><strong>Med gene mutation and G6PD silent gene mutation </strong><strong>using real-time PCR. </strong><strong><em>Results:</em></strong><strong> The current study shows the incidence of G6PD</strong><strong>Med gene mutation in 26 patients (5</strong><strong>2</strong><strong>%) and that 96.2% of G6PD patients with G6PD Med gene mutation had G6PD silent gene mutation. </strong><strong><em>Conclusion:</em></strong><strong> The current study shows the incidence of G6PD Med gene mutation in 5</strong><strong>2</strong><strong>% of patients and that 96.2% of G6PD patients with Med Gene mutation had G6PD silent gene mutation. </strong><strong>The presence of either G6PD Med gene mutation or both G6PD Med gene mutation and G6PD silent gene mutation together were insignificant in the prediction of G6PD status whether diseased or </strong><strong>not. G6PD deficiency could be a consequence of a complex multifactorial mechanism probably related to both environmental factors and genetic modifiers.</strong><br /> https://scumj.journals.ekb.eg/article_342466_558401980e7735ba2ec5f8f701947d6f.pdf