Suez Canal University, Faculty of MedicineSuez Canal University Medical Journal1110-699922220191001Impact of Serum Interleukin 6 among Helicobacter Pylori-Positive Adult Patients in Relation to upper gastrointestinal endoscopy findings1171219480310.21608/scumj.2019.94803ENSeham OmarDepartment of Internal Medicine, Faculty of Medicine, Suez Canal University, EgyptMohamed FHassanDepartment of Endemic and Infectious Diseases, Faculty of Medicine, Suez Canal University,Egypt.Basma BHasanDepartment of Clinical Pathology, Faculty of Medicine, Port Said University, EgyptJournal Article20200610<strong><em>Background: </em></strong><strong>Helicobacter pylori (H. pylori) is one of the most common chronic bacterial infections in humans and has a major etiologic role for several gastroduodenal diseases. Interleukin-6 (IL-6) is a multifunctional cytokine and </strong><strong>H. Pylori may stimulate its production. <em>Aim:</em> To assess the serum level of IL-6 in H. pylori-infected patients and to study its relation to gastric endoscopy findings. Subjects and <em>Patients and Methods</em>: Eighty-four participants were enrolled; 42 patients with positive H. pylori (patient group “B”), and 42 age and gender-matched subjects with negative H. pylori (control group “A”). Out of 42 patients in group B, 21 patients were subjected to upper gastrointestinal endoscopy due to persistent gastric upset. The serum</strong><strong> concentration of IL-6 was measured in all subjects by ELISA</strong><strong>. <em>Results:</em> serum </strong><strong>IL-6 was significantly higher in patients’ group compared to control group (4.3 ± 0.7 <em>vs</em> 1.5 ± 0.3 pg/ml, <em>p</em> <0.05). The prevalence of IL-6 in infected patients with H. Pylori was 92%, with the highest levels in those with gastric ulcers. <em>Conclusion</em>: H. Pylori infection is associated with high serum levels of IL-6 especially in those with gastric ulcers. </strong><br /> <strong><em>Keywords:</em></strong> <em>Helicobacter pylori; interleukin 6</em>https://scumj.journals.ekb.eg/article_94803_cbddfc20f6b45b3e19b1ba462b725a46.pdfSuez Canal University, Faculty of MedicineSuez Canal University Medical Journal1110-699922220191001Urinary Monocyte Chemoattractant Protein -1 as A Diagnostic Marker of Lupus Nephritis1221309497610.21608/scumj.2019.94976ENGamal ATawfikDepartment of Internal Medicine, Nephrology Unit, Suez Canal University, Faculty of Medicine, EgyptEman AGhareebDepartment of Internal Medicine, Nephrology Unit, Suez Canal University, Faculty of Medicine, EgyptWaleed OAbd El-WaheedDepartment of Internal Medicine, Nephrology Unit, Suez Canal University, Faculty of Medicine, EgyptMohammed MKeshawyDepartment of Internal Medicine, Nephrology Unit, Suez Canal University, Faculty of Medicine, EgyptHeba MZakiDepartment of Internal Medicine, Nephrology Unit, Suez Canal University, Faculty of Medicine, EgyptJournal Article20200611<strong><em>Background: </em></strong><strong>Lupus nephritis accounts for significant morbidity and mortality in patients with systemic lupus erythematosus (SLE). Many biomarkers for renal involvement in SLE have been suggested in Egyptian patients; one of them is Monocyte chemoattractant protein-1 (MCP-1) which is one of the key chemokines that have chemotactic effect for monocytes and macrophages to sites of inflammation and may share in the pathogenesis of lupus nephritis (LN). </strong><strong><em>Aim:</em></strong><strong> The study aimed at assessing the role of MCP-1 in the early diagnosis of lupus nephritis and to explore any correlation its levels with disease activity and renal status. </strong><strong><em>Subjects and Methods:</em></strong><strong> The study was done as a case-control study where 60 SLE patients with lupus nephritis (30 patients with active LN and 30 patients with inactive LN) in addition to 30 healthy volunteers as control group were enrolled in the study and MCP-1 levels was determined using ELISA technique. <em>Results:</em> </strong><strong>Urinary MCP-1 levels in SLE studied patients’ groups were significantly higher than their level in the control group (</strong><strong>p = 0.0001)</strong><strong> and it was significantly higher in active LN than in non-active LN subgroups (P-value =0.0001). </strong><strong><em>Conclusion</em></strong><strong>: Urinary MCP-1 can be used as a marker for LN activity.</strong><br /> <strong>Keywords: </strong>SLE, Lupus nephritis, MCP-1https://scumj.journals.ekb.eg/article_94976_6938bc3788699de2ae8c0eb0f7e03bd2.pdfSuez Canal University, Faculty of MedicineSuez Canal University Medical Journal1110-699922220191001Assessment of the Platelet Adenosine Diphosphate Receptor (ADP Receptor p2y12) Polymorphism in Coronary Heart Diseases Patients in Ismailia1311379497710.21608/scumj.2019.94977ENAhmed AMohamedDepartment of Clinical Pathology, Faculty of Medicine, Suez Canal University.Alaa Eldin SAbdelhamidDepartment of Clinical Pathology, Faculty of Medicine, Suez Canal University.Hesham HIbrahimDepartment of Cardiology, Faculty of Medicine, Suez Canal University.Amany MHassanDepartment of Clinical Pathology, Faculty of Medicine, Suez Canal University.Journal Article20200611<strong><em>Background</em></strong><strong>: Cardiovascular diseases are the leading cause of death in developed countries. In Egypt mortality rates from coronary heart disease are one of the highest worldwide, also coronary heart diseases will be the leading cause of death in developing countries by the year 2020. P2Y12 ADP receptor gene polymorphism is now considered one important implication factor in atherothrombosis and coronary heart diseases. <em>Aim: </em>To assess the correlation between the platelet Adenosine Diphosphate receptor (P2Y12) polymorphism and coronary heart disease patients in Ismailia. <em>Subject and Methods: </em>An analytical case-control study; conducted on 100 volunteers divided into 2 groups; 50 for study (patient) group and 50 for the control group at Ismailia Hospitals' Cardiology premises (clinics & inpatient department) to assess the correlation between the platelet ADP receptor (P2Y12) polymorphism and coronary heart diseases in Ismailia. The patients were subjected to an Interview Questionnaire, clinical examination, and Laboratory Investigations and testing for P2Y12 gene polymorphism. <em>Results: </em>In the study group, 42% of patients were hypertensive, 48% were diabetics, 60% were dyslipidemic and 66% were smokers. In the control group individuals, 26% were hypertensive, 20% were diabetics, 12% were dyslipidemic and 24% were smokers. DNA extraction and examination for P2Y12 gene polymorphism were negative in all participants. <em>Conclusion: </em>Our study revealed that there is no correlation between the platelet ADP receptor (P2Y12) gene polymorphism and coronary heart diseases in Ismailia.</strong><br /> <strong>Keywords</strong>: Ischemic heart disease, Thrombophilia, Platelet glycoproteins, Atherothrombosishttps://scumj.journals.ekb.eg/article_94977_d604c5e083633e39ffa812f269ca79e4.pdfSuez Canal University, Faculty of MedicineSuez Canal University Medical Journal1110-699922220191001Isolation and Characterization of Mesenchymal Stem Cells from Human Liposuction Aspirate1381459498110.21608/scumj.2019.94981ENAlaa Eldin SAbdelhamidDepartment of Clinical Pathology, Faculty of Medicine, Suez Canal University.Mohamed SEl ShahedyDepartment of Virology, Faculty of Veterinarian Medicine, Suez Canal University.Mona HMohammedDepartment of Anatomy and Embryology, Faculty of Medicine, Suez Canal University.Ghada SAlyDepartment of Anatomy and Embryology, Faculty of Medicine, Suez Canal University.Bassma MDessoukiDepartment of Anatomy and Embryology, Faculty of Medicine, Suez Canal University.Journal Article20200611<strong><em>Background:</em></strong><strong> Adipose derived stem cells (ADSCs) are a new type of mesenchymal stem cells (MSC) resemble bone marrow stem cells in their biologic activities. DScs is multipotent stem cells can differentiate into multiple cell types including osteoblasts, chondrocytes, adipocytes, myocytes, vascular endothelial cells, and neurons. Stromal vascular fraction (SVF) corresponds to ADSC and describes the cells obtained immediately from adipose tissues after the digestion of collagenase. Adipose tissue is a promising source of adult mesenchymal stem cells for therapeutic applications because it is available in large amounts (100 ml up to 1 liter) through liposuction and with minimal morbidity. <em>Aim:</em> </strong><strong>To isolate and characterize the adipose-derived stem cells from human liposuction aspirate. <em>Material and </em></strong><strong><em>Methods: </em></strong><strong>liposuction aspirate was used for the extraction of the stromal vascular fragment (SVF). SVF was used for culturing and isolation of hADSCs.</strong><strong>ADSCs were cultured in tissue-culture flasks using collagenase digestion method. Flow cytometric analysis was used to identify ADSCs markers using fluorescence-activated cell sorting (FACS)</strong><strong>against CD44, CD90, and CD45. </strong><strong><em>Results: </em></strong><strong>Human ADSCs were isolated successfully using collagenase digestion method. After 4 days the cells appeared to be spindle in shape and formed symmetric colonies. After 7 days, the cell sheet formed 60% confluence and completed to 90% confluence after 10 days incubation. FACS analysis of ADSCs surface markers revealed expression of CD44, and CD90 markers in most of the adherent cells. In contrast, most adherent cells were negative for CD45. </strong><strong><em>Conclusion:</em></strong><strong> Human ADSCs were isolated successfully from human lipoaspirate.</strong><br /> <strong><em>Keywords:</em></strong> ADSCs; MSC; SVFhttps://scumj.journals.ekb.eg/article_94981_1011e2a349f1b80bda20e4be7605077d.pdfSuez Canal University, Faculty of MedicineSuez Canal University Medical Journal1110-699922220191001Circulating sTWEAK Levels and Diabetic Nephropathy in Type 2 Diabetic Patients1461569799610.21608/scumj.2019.97996ENSara AHabashDepartment of Clinical Pathology, Faculty of Medicine, Suez Canal University, EgyptMohamed OAbdallaDepartment of Clinical Pathology, Faculty of Medicine, Suez Canal University, EgyptFekry GEskandarDepartment of Clinical Pathology, Faculty of Medicine, Suez Canal University, EgyptNashwa RHassanDepartment of Clinical Pathology, Faculty of Medicine, Suez Canal University, EgyptJournal Article20200625<strong><em>Background</em></strong><strong>: </strong><strong>Diabetic nephropathy </strong><strong>(DN) is one of the major complications of </strong><strong>diabetes mellitus (DM) </strong><strong>that leads to end stage renal disease (ESRD)</strong><strong>.</strong><strong> There is a need for novel biomarkers for early detection and follow up of patients with DN. </strong><strong>TNF-like weak inducer of apoptosis </strong><strong>(TWEAK) is thought to be involved </strong><strong>in the progression of DN through the fibroblast growth factor inducible-14 (Fn14)</strong><strong> pathway. Accordingly, blood soluble form of TWEAK (sTWEAK) could be a marker for the progression of and degree of DN. <em>Aim</em>:</strong><strong> To assess the relationship between circulating sTWEAK levels and DN in type 2 diabetic patients as it could be a good potential biomarker for detection and follow up of DN patients. </strong><strong><em>Patients and Methods:</em></strong><strong>Sixty</strong><strong> type 2 DM patients were selected to be 30 with </strong><strong>DN and 30 without DN. DN patients were further divided into 20 microalbuminuric and 10 macro-albuminuric patients. Twenty normal subjects served as a control group. Patients were tested for serum creatinine, lipid prolife, glycated hemoglobin, urinary albumin creatinine ratio (ACR), estimated glomerular filtration rate (eGFR) and serum sTWEAK. </strong><strong><em>Results:</em></strong><strong> Serum sTWEAK was found to change significantly with the level of albuminuria with its lowest level in the macro-albuminuric group (</strong><strong>P <0.0001</strong><strong>). There was a significant positive correlation between serum sTWEAK and eGFR (r =0.315, p- value =</strong><strong><em>0.014</em></strong><strong>). Furthermore, sTWEAK showed good diagnostic value for detection of macro-albuminuria, with 90% sensitivity and 70% Specificity. </strong><strong><em>Conclusion:</em></strong><strong> Serum sTWEAK could be a good biomarker for detection and follow up of DN. </strong>https://scumj.journals.ekb.eg/article_97996_ba0dbdc6c5f2ab7d6d15605805e7876d.pdfSuez Canal University, Faculty of MedicineSuez Canal University Medical Journal1110-699922220191001Serum Immunoglobulins in Children with Vitamin D Resistant Rickets15716311050710.21608/scumj.2019.110507ENEzzat AminDepartment of Pediatrics's, Zagazig University, Egypt0000-0002-2329-6073Hossam KamalDepartment of Pediatrics's, Zagazig University, EgyptAhmed Gab-AllahDepartment of Clinical Pathology, Tarablus University Hospital, LibyaSeham AlzarrouqDepartment of Pediatrics's, Tarablus University Hospital, LibyaMohamed ATalatDepartment of Pediatrics's, Zagazig University, Egypt0000-0002-1548-5720Journal Article20200901<strong><em>Background:</em></strong><strong> Vitamin D plays an immunoregulatory role in both the innate and adaptive immune systems. Rickets is a disorder that can develop due to a lack of vitamin D, calcium, or phosphate. <em>Aim:</em> The aim of this study was to assess serum immunoglobulins as one of the aspects of humoral immunity in children with vitamin D resistant rickets. <em>Subjects and Methods: </em>This case-control study was performed on 60 patients divided into Group (A):30 cases with vitamin D resistant rickets patients who were presented with rachitic manifestation who didn’t respond to therapeutic doses of the vitamin. Group (B):30 cases with untreated classic vitamin D deficiency rickets. Group (C): 30 healthy subjects as a control group. All subjects were subjected to detailed history taking including age, sex, weight, dietary history, medication history, and physical examination including vital signs, body weight, and head circumference. Laboratory investigations that included calcium, phosphorus, alkaline phosphatase, electrolytes, ESR, urine analysis, parathyroid hormone, vitamin D, and Immunoglobulin assay. <em>Results:</em> There is a significant decrease of IgA in vitamin D deficiency rickets and vitamin D resistant rickets than in controls (3.74 ± .867, 3.13 ± 1.72, 5.01 ± 1.61, respectively with P =0.012).There is a significant decrease of IgG in vitamin D deficiency rickets and a significant increase in vitamin D resistant rickets than in controls (523.5 ± 136.3, 751.25 ±163.5, 692.0 ± 186.4, respectively with P =0.009). There is no significant difference in IgM in the three studied groups. <em>Conclusion:</em> </strong><strong>Patients with vitamin D deficiency have significantly lower IgG levels, while vitamin D resistant patients have significantly higher IgG levels. Whereas, both groups have lower IgA and normal IgM levels.</strong>https://scumj.journals.ekb.eg/article_110507_7a5fc390b52031b0eb0fee81d06e0c8b.pdfSuez Canal University, Faculty of MedicineSuez Canal University Medical Journal1110-699922220191001Evaluation of Staging Laparoscopy in Colorectal Cancer Assessment16416911604710.21608/scumj.2019.116047ENAhmed HassanDepartment of General Surgery, Faculty of Medicine, Suez Canal University, Ismailia, EgyptMohammed ElkerkaryDepartment of General Surgery, Faculty of Medicine, Suez Canal University, Ismailia, EgyptMohamed GamalDepartment of General Surgery, Faculty of Medicine, Suez Canal University, Ismailia, EgyptMahmoud GamalDepartment of General Surgery, Faculty of Medicine, Suez Canal University, Ismailia, EgyptAhmed GomaaDepartment of General Surgery, Faculty of Medicine, Suez Canal University, Ismailia, EgyptIslam KhaledDepartment of General Surgery, Faculty of Medicine, Suez Canal University, Ismailia, Egypt0000-0001-6577-4312Journal Article20200929<strong><em>Background:</em></strong><strong> The goal of staging laparoscopy in primary gastrointestinal malignancies is to examine any local, regional, and/or metastatic disease in order to determine the stage of the presenting malignancy, and thus, the possibility of performing a curative resection. This study aimed to evaluate the role of staging laparoscopy in preoperative assessment of colorectal cancer patients. <em>Subjects</em></strong><strong><em> and Method:</em></strong><strong>this is a cross-sectional study, conducted at Suez Canal University hospital. A total of 26 patients with colorectal cancer were enrolled and had a CT scanning with contrast and staging laparoscopy as a step in the preoperative assessment of their disease. <em>Results:</em> Staging laparoscopy of these patients revealed metastatic spread in 10 cases (38%). Both pelvi-abdominal CT and staging laparoscopy had equally high specificity for metastatic diseases (99%). However, regarding the sensitivity, staging laparoscopy was superior to pelvi-abdominal CT in detecting the presence of metastatic disease in general (83% <em>vs</em> 66%, p=0.026). <em>Conclusion</em>: staging laparoscopy is a valuable tool in evaluating colorectal cancer patients preoperatively. Future studies should be conducted on a higher number of patients in order to detect the actual value of staging laparoscopy in detecting metastatic spread. </strong>https://scumj.journals.ekb.eg/article_116047_eefac2549dde3f0438ebfb54666f8dfa.pdfSuez Canal University, Faculty of MedicineSuez Canal University Medical Journal1110-699922220191001The Validity of Modified Alvarado Scoring System as a Predictor Tool for Diagnosis of Acute Appendicitis in a Low-income Country; a Comparative Prospective Study17017811062410.21608/scumj.2019.110624ENGad MBehairyDepartment of General Surgery, Ain Shams University Hospitals, Cairo, EgyptAbdelrahman MElghandourDepartment of General Surgery, Ain Shams University Hospitals, Cairo, EgyptIslam KhaledDepartment of General Surgery, Suez Canal University Hospitals, Ismailia, Egypt0000-0001-6577-4312Journal Article20200902<strong><em>Background</em></strong><strong><em>:</em></strong><strong> Acute appendicitis is a disease with a well-known long history. The final diagnosis of acute appendicitis is only accurate with histopathological findings after appendectomy. However, the choice to carry out surgical treatment is based exclusively on clinical assessment which does not reach the precise diagnosis of appendicitis. Therefore, Different scoring systems have emerged in order to help physicians in decision making and diagnosis of suspicious individuals. <em>Aim:</em> To determine the diagnostic accuracy of the Modified Alvarado Scoring System in a low-income country in the diagnosis of appendicitis. </strong><strong><em>Patients and Methods:</em></strong><strong> This was a cross-sectional study that included 200 patients with right iliac fossa pain, suggestive of acute appendicitis. The patients were examined clinically and randomly allocated into 2 groups. The Modified Alvarado Scoring System (MASS) was calculated for the case group and listed and compared to another control group for which the operative decision was based on the clinical evaluation without relying on a scoring system. All the studied patients went through appendectomy according to overall clinical judgment and never based on MASS only. Specimens were sent to histopathological examination to verify the diagnosis. </strong><strong><em>Results:</em></strong><strong> The results showed that MASS at the cut-off value of ≥ 7 has a sensitivity of 93.33%, a specificity of 52.94%, and accuracy of 84.42%, and a negative appendectomy rate of 17 out of 95 patients (17.8%). <em>Conclusion:</em> MASS is a safe tool in the diagnosis of acute appendicitis due to its accepted sensitivity and higher specificity</strong><strong>. It could be helpful in low-income countries, where resources are limited.</strong>https://scumj.journals.ekb.eg/article_110624_794591b6d89b584cb4bca5c346b8b736.pdfSuez Canal University, Faculty of MedicineSuez Canal University Medical Journal1110-699922220191001Gene Xpert MTB/RIF Assay as A New Tool for Tuberculosis Diagnosis and Detection of Rifampin Resistance17918611068010.21608/scumj.2019.110680ENRabab Al OlimeyDepartment of Chemical and Clinical Pathology, Faculty of Medicine, Benha University, EgyptSohier AbdElSamieDepartment of Chemical and Clinical Pathology, Faculty of Medicine, Benha University, EgyptMohamed KamelDepartment of Internal Medicine (Chest), Faculty of Medicine, Benha University, EgyptAfaf KhamisDepartment of Chemical and Clinical Pathology, Faculty of Medicine, Benha University, EgyptJournal Article20200902<strong><em>Background:</em></strong><strong> Mycobacterium tuberculosis (MTB) </strong><strong>is one of the top ten causes of death and the leading cause of a single infectious agent (above HIV/AIDS) Worldwide. Each year millions of people continue to fall sick with TB. </strong><strong><em>Aim: </em></strong><strong>To evaluate the MTB/RIF assay for rapid diagnosis and detection of rifampin resistance of tuberculosis in both positive and negative smears-as well as pulmonary and non-pulmonary clinical specimens. <em>Patients and Methods: </em></strong><strong>A prospective study was conducted on 50 patients (37 pulmonary, 13 extrapulmonary) patients attending the Outpatient Clinic and inpatients of Chest Department, Benha University Hospital, Egypt. P</strong><strong>ulmonary samples</strong><strong> were </strong><strong>analyzed by (Sputum smear exam. by Ziehl Neelsen (ZN) stain, culture on </strong><em><strong>Lowenstein</strong></em><strong>-</strong><em><strong>Jensen</strong></em><strong>(LJ)</strong><strong>media, and assessment of rifampin sensitivity). Extrapulmonary samples were analyzed by reference methods (pathological diagnosis) and by GeneXpert. <em>Results:</em> </strong><strong>pulmonary patients’ smears were 32 (86.5%) positive and 5 (13.5%) negative. Culture on</strong><strong> LJ</strong><strong>revealed rifampin sensitivity in 33/37 (89.1%) and rifampin-resistant in 4/37 (10.9%), GeneXpert was positive in 32(86.5%) and negative in 5 (13.5%), rifampin assay was sensitive in 33 (89.1%) and resistant in 4(10.9%). All extrapulmonary patients, (n=13) were positive on GeneXpert and sensitive for rifampin. Conclusion:</strong><strong> GeneXpert could be considered as a leading way to early diagnosis, treatment, and prevention of transmission of tuberculosis which could reduce TB- associated morbidity and mortality.</strong>https://scumj.journals.ekb.eg/article_110680_4ed7516bb97e75bcf568c47334d9501d.pdfSuez Canal University, Faculty of MedicineSuez Canal University Medical Journal1110-699922220191001Role of Staging Laparoscopy in Assessing Colorectal Cancer16416926269910.21608/scumj.2020.23076.1007ENAhmed HassanSolimanDepartment of Surgery, Faculty of Medecine, Suez Canal universityMohammed GamalSayeddepartement of surgery, faculty of medecine, sue canal universityJournal Article20200127Background: The goal of staging laparoscopy in primary gastrointestinal malignancies is to examine any local, regional, and/or metastatic disease in order to determine the stage of the presenting malignancy, and thus, the possibility of performing a curative resection. This study aimed to evaluate the role of staging laparoscopy in preoperative assessment of colorectal cancer patients.<br /> <br /> Subjects and Method: this is a cross-sectional study, conducted at Suez Canal University hospital. A total of 26 patients with colorectal cancer were enrolled and had a CT scanning with contrast and staging laparoscopy as a step in the preoperative assessment of their disease.<br /> <br /> Results: Staging laparoscopy of these patients revealed metastatic spread in 10 cases (38%). Both pelvi-abdominal CT and staging laparoscopy had equally high specificity for metastatic diseases (99%). However, regarding the sensitivity, staging laparoscopy was superior to pelvi-abdominal CT in detecting the presence of metastatic disease in general (83% vs 66%, p=0.026).<br /> <br /> Conclusion: staging laparoscopy is a valuable tool in evaluating colorectal cancer patients preoperatively. Future studies should be conducted on a higher number of patients in order to detect the actual value of staging laparoscopy in detecting metastatic spread.